Journal: Scientific Reports

Article Title: Quantitative Model for Ion Transport and Cytoplasm Conductivity of Chinese Hamster Ovary Cells

doi: 10.1038/s41598-018-36127-3

Figure Lengend Snippet: Comparison of the cytoplasm conductivity of various cells in different physiological states.

Article Snippet: The ratio of low conductivity to BioGro CHO medium was adjusted to reach the desired media conductivity 0.42 S/m.

Techniques: Comparison, Cell Differentiation, Inhibition, Incubation

Journal: Scientific Reports

Article Title: Quantitative Model for Ion Transport and Cytoplasm Conductivity of Chinese Hamster Ovary Cells

doi: 10.1038/s41598-018-36127-3

Figure Lengend Snippet: Simulated spectrum of the real part of the Claussius-Mossotti factor (Re {K CM }) for a mammalian cell (Chinese hamster ovary (CHO)), with parameters from . ( a ) Cytoplasm conductivity varies from 0.35–0.5 S/m and medium conductivity is 0.42 S/m. ( b ) Cell volume varies from 5 × 10 −10 –10 × 10 −10 cm 3 and medium conductivity is 0.42 S/m.

Article Snippet: The ratio of low conductivity to BioGro CHO medium was adjusted to reach the desired media conductivity 0.42 S/m.

Techniques:

Journal: Scientific Reports

Article Title: Quantitative Model for Ion Transport and Cytoplasm Conductivity of Chinese Hamster Ovary Cells

doi: 10.1038/s41598-018-36127-3

Figure Lengend Snippet: Simulation and experimental results of pump inhibited CHO cells using 5 mM Ouabain. The marked lines show the estimated cytoplasm conductivity using the experimental results of three DEP measurements for 105 min. Each marker represents the average for 5 min intervals (100–150 cells). The solid line represents the simulation results of the pump inhibited CHO cells. To inhibit the pumps, pump density is set to zero.

Article Snippet: The ratio of low conductivity to BioGro CHO medium was adjusted to reach the desired media conductivity 0.42 S/m.

Techniques: Marker